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Mesophyll protoplasts of these test plants were isolated from the in vitro-grown young seedlings by treatment of the enzyme solution containing 2 % Cellulase Onozuka RS, 0.5 % Cellulase Onozuka R-10,0.5 % Macerozyme R-10,0.02 % Pectolyase Y-23,CPW salts, 0.1 % MES and 0.5 M sorbitol (pH 5.8) for 4-11 hours. 2. The cell division and colony formation of spinach cv. \u0027SHINNIPPON\u0027 occurred only in a modified MS medium (No. 3) containing 1 mg/l NAA, 1 mg/l 2,4-D and 1 mg/l BA in five combinations of growth regulators. 3. The cell division and colony formation of protoplasts only occurred in all spinach cvs., but not in chard and tablebeet in No. 3 medium. 4. The frequency of cell division and colony formation in spinach cv. \u0027F_1 SHINRYOKU\u0027 increased to DMSO concentration. Plating efficiency of the medium containing 1.0 % DMSO (14.7 %) was much higher than that of the medium without DMSO (3.2 %) . 5. We now plan to induce plant regeneration from the thus obtained callus.", "subitem_description_type": "Other"}]}, "item_1_source_id_13": {"attribute_name": "雑誌書誌ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN10463454", "subitem_source_identifier_type": "NCID"}]}, "item_1_text_10": {"attribute_name": "著者所属(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Dept. Horticulture, Keisen Jogakuen Junior College"}, {"subitem_text_language": "en", "subitem_text_value": "Faculty of Agriculture, Iwate University"}]}, "item_1_text_9": {"attribute_name": "著者所属(日)", "attribute_value_mlt": [{"subitem_text_value": "恵泉女学園短期大学園芸生活学科"}, {"subitem_text_value": "岩手大学農学部"}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "1998-03-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00000198960.pdf", "filesize": [{"value": "1.6 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 1600000.0, "url": {"label": "ホウレンソウ葉肉プロトプラストの初期培養条件の改良 ", "url": "https://keisen.repo.nii.ac.jp/record/656/files/KJ00000198960.pdf"}, "version_id": "83dc7861-5845-4d51-96d6-0bb295edca08"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "ホウレンソウ葉肉プロトプラストの初期培養条件の改良", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "ホウレンソウ葉肉プロトプラストの初期培養条件の改良"}, {"subitem_title": "Improvement of Plating Efficiency on the Mesophyll Protoplast Culture of Spinach, Spinacia oleracea L.", "subitem_title_language": "en"}]}, "item_type_id": "1", "owner": "3", "path": ["89"], "permalink_uri": "https://keisen.repo.nii.ac.jp/records/656", "pubdate": {"attribute_name": "公開日", "attribute_value": "1998-03-01"}, "publish_date": "1998-03-01", "publish_status": "0", "recid": "656", "relation": {}, "relation_version_is_last": true, "title": ["ホウレンソウ葉肉プロトプラストの初期培養条件の改良"], "weko_shared_id": 3}