{"created":"2023-06-19T06:53:10.863294+00:00","id":656,"links":{},"metadata":{"_buckets":{"deposit":"95ca609a-98ec-4576-a711-128ae8736c0d"},"_deposit":{"created_by":3,"id":"656","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"656"},"status":"published"},"_oai":{"id":"oai:keisen.repo.nii.ac.jp:00000656","sets":["74:89"]},"author_link":["1736","1686","1690","1318","1735","1734","1737","1738"],"item_1_alternative_title_5":{"attribute_name":"論文名よみ","attribute_value_mlt":[{"subitem_alternative_title":"ホウレンソウ ハニク プロトプラスト ノ ショキ バイヨウ ジョウケン ノ カイリョウ"}]},"item_1_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1998-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"20","bibliographicPageStart":"15","bibliographicVolumeNumber":"29","bibliographic_titles":[{"bibliographic_title":"研究紀要 : 恵泉女学園短期大学園芸生活学科"},{"bibliographic_title":"Research Bulletin : Published by Dept. Horticulture, Keisen Junior College","bibliographic_titleLang":"en"}]}]},"item_1_creator_6":{"attribute_name":"著者名(日)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"藤田, 智"},{"creatorName":"フジタ, サトシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"1690","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000060209054","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000060209054"}]},{"creatorNames":[{"creatorName":"杉山, 信太郎"},{"creatorName":"スギヤマ, シンタロウ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"1686","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000020162901 ","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000020162901 "}]},{"creatorNames":[{"creatorName":"遠藤, 元庸"},{"creatorName":"エンドウ, モトノブ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"1734","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000050021697","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000050021697"}]},{"creatorNames":[{"creatorName":"稲田, 委久子"},{"creatorName":"イナダ, イクコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"1735","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000090110650","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000090110650"}]}]},"item_1_creator_8":{"attribute_name":"著者名(英)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"FUJITA,, Satoshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1318","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000060209054","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000060209054"}]},{"creatorNames":[{"creatorName":"SUGIYAMA,, Shintaro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1736","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000050021697","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000050021697"}]},{"creatorNames":[{"creatorName":"ENDO,, Motonobu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1737","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000090110650","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000090110650"}]},{"creatorNames":[{"creatorName":"INADA,, Ikuko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1738","nameIdentifierScheme":"WEKO"}]}]},"item_1_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_1_description_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"In order to improve the plating efficiency on spinach mesophyll protoplast culture, various conditions were examined with four cvs. of spinach, four cvs. of chard and one cv. of tablebeet. 1. Mesophyll protoplasts of these test plants were isolated from the in vitro-grown young seedlings by treatment of the enzyme solution containing 2 % Cellulase Onozuka RS, 0.5 % Cellulase Onozuka R-10,0.5 % Macerozyme R-10,0.02 % Pectolyase Y-23,CPW salts, 0.1 % MES and 0.5 M sorbitol (pH 5.8) for 4-11 hours. 2. The cell division and colony formation of spinach cv. 'SHINNIPPON' occurred only in a modified MS medium (No. 3) containing 1 mg/l NAA, 1 mg/l 2,4-D and 1 mg/l BA in five combinations of growth regulators. 3. The cell division and colony formation of protoplasts only occurred in all spinach cvs., but not in chard and tablebeet in No. 3 medium. 4. The frequency of cell division and colony formation in spinach cv. 'F_1 SHINRYOKU' increased to DMSO concentration. Plating efficiency of the medium containing 1.0 % DMSO (14.7 %) was much higher than that of the medium without DMSO (3.2 %) . 5. We now plan to induce plant regeneration from the thus obtained callus.","subitem_description_type":"Other"}]},"item_1_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN10463454","subitem_source_identifier_type":"NCID"}]},"item_1_text_10":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Dept. Horticulture, Keisen Jogakuen Junior College"},{"subitem_text_language":"en","subitem_text_value":"Faculty of Agriculture, Iwate University"}]},"item_1_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"恵泉女学園短期大学園芸生活学科"},{"subitem_text_value":"岩手大学農学部"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"1998-03-01"}],"displaytype":"detail","filename":"KJ00000198960.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ホウレンソウ葉肉プロトプラストの初期培養条件の改良 ","url":"https://keisen.repo.nii.ac.jp/record/656/files/KJ00000198960.pdf"},"version_id":"83dc7861-5845-4d51-96d6-0bb295edca08"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ホウレンソウ葉肉プロトプラストの初期培養条件の改良","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ホウレンソウ葉肉プロトプラストの初期培養条件の改良"},{"subitem_title":"Improvement of Plating Efficiency on the Mesophyll Protoplast Culture of Spinach, Spinacia oleracea L.","subitem_title_language":"en"}]},"item_type_id":"1","owner":"3","path":["89"],"pubdate":{"attribute_name":"公開日","attribute_value":"1998-03-01"},"publish_date":"1998-03-01","publish_status":"0","recid":"656","relation_version_is_last":true,"title":["ホウレンソウ葉肉プロトプラストの初期培養条件の改良"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-19T07:06:13.554091+00:00"}